《植物生理学报》 2015, 51(3): 317-322

不同春石斛品种扦插苗茎尖激素含量与其成花品质关系

贾梦雪, 樊翠霞, 狄玮, 邹成勇, 刘燕*

北京林业大学园林学院, 国家花卉工程技术研究中心, 花卉种质创新与分子育种北京市重点实验室, 城乡生态环境北京实验室, 北京100083

通信作者：刘燕；E-mail: chblyan@163.com；Tel: 010-62336062

摘　要：

为探讨春石斛扦插苗生长发育过程中内源激素与成花品质的关系, 本研究以成花品质不同的2个品种‘森禾H1’和‘森禾4001’为试材, 对其一年生扦插苗生长发育过程中5个不同阶段茎尖内源激素含量进行了比较分析。结果显示: 整个生长发育过程中, 2个品种扦插苗茎尖中赤霉酸(GA₃)、吲哚乙酸(IAA)、脱落酸(ABA)含量以及GA₃/IAA、GA₃/ABA、(GA₃+IAA)/ABA变化趋势相似。成花品质较好的‘森禾H1’, 其GA₃、GA₃/IAA、GA₃/ABA、(GA₃+IAA)/ABA, 除了在休止叶后期外, 其他时期均一直显著高于成花品质较差的‘森禾4001’, 而其ABA含量在萌动期、展叶期、旺盛生长期显著低于‘森禾4001’, IAA含量没有显示出规律性。结果表明, 春石斛扦插苗的一些内源激素含量及比例与其后期成花品质密切相关, 可作为优良品种早期筛选的参考指标。

关键词：春石斛; 内源激素; 扦插苗; 品种筛选

收稿：2014-12-04   修定：2015-01-19

资助：国家“十二五”支撑计划课题(2011BAD12B02-01)。

The Relation between Endogenous Hormones in Shoot Tips of Nobile-Type Dendrobium Young Cutting Plant and Flowering Quality

JIA Meng-Xue, FAN Cui-Xia, DI Wei, ZOU Cheng-Yong, LIU Yan*

Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing 100083, China

Corresponding author: LIU Yan; E-mail: chblyan@163.com; Tel: 010-62336062

Abstract:

The aim is to explore the relations between flowering quality and endogenous hormones in growth process of nobile-type Dendrobium young cutting plants. Hormones in shoot tips at five growth stages from two nobile-type Dendrobium cultivars, D. ‘Senhe H1’ and D. ‘Senhe 4001’, with different flowering quality, were studied comparatively. The results showed that changes of gibberellin acid (GA₃), indole-3-acetic acid (IAA), abscisic acid (ABA), GA₃/IAA, GA₃/ABA, (GA₃+IAA)/ABA in shoot tips of two cultivars were similar. But GA₃, GA₃/IAA, GA₃/ABA, (GA₃+IAA)/ABA from ‘Senhe H1’ with a better flowering quality were always significantly higher, except the later period of resting leaf appearing. Whereas the ABA contents of ‘Senhe 4001’ with a bad flowering quality were significant higher at sprouting, leaf-expansion and vigorous growth stages. IAA content showed no regularity in variation. These results indicated that some hormonal contents and ratios were closely related to the flowering quality, which could be used as reference indexes for early screening of elite cultivars.

Key words: nobile-type Dendrobium; endogenous hormones; cuttings; cultivars screening